2-Nitrophenyl beta-D-galactopyranoside, >=98% (enzymatic)

2-Nitrophenyl beta-D-galactopyranoside, >=98% (enzymatic)

Application

2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.

Biochem/physiol Actions

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

General description

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Packaging

500 mg in poly bottle

250 mg in glass bottle

1, 5, 25 g in poly bottle

Principle

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Reconstitution

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

Substrates

Chromogenic substrate for β-galactosidase

No detailed specifications are available for this product.

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Code Description Quality Level InChI key InChI Manufacturer Storage Temp. Form Assay Price Quantity
3577216 2-Nitrophenyl beta-D-galactopyranoside, >=98% (enzymatic) 200 KUWPCJHYPSUOFW-YBXAARCKSA-N 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 SIGMA-ALDRICH −20°C powder ≥98% (enzymatic)
£67.69 (exc VAT) per 1G
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3577217 2-Nitrophenyl beta-D-galactopyranoside, >=98% (enzymatic) 200 KUWPCJHYPSUOFW-YBXAARCKSA-N 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 SIGMA-ALDRICH −20°C powder ≥98% (enzymatic)
£34.68 (exc VAT) per 250MG
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3577218 2-Nitrophenyl beta-D-galactopyranoside, >=98% (enzymatic) 200 KUWPCJHYPSUOFW-YBXAARCKSA-N 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 SIGMA-ALDRICH −20°C powder ≥98% (enzymatic)
£922.07 (exc VAT) per 25G
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3577219 2-Nitrophenyl beta-D-galactopyranoside, >=98% (enzymatic) 200 KUWPCJHYPSUOFW-YBXAARCKSA-N 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 SIGMA-ALDRICH −20°C powder ≥98% (enzymatic)
£46.38 (exc VAT) per 500MG
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3577220 2-Nitrophenyl beta-D-galactopyranoside, >=98% (enzymatic) 200 KUWPCJHYPSUOFW-YBXAARCKSA-N 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 SIGMA-ALDRICH −20°C powder ≥98% (enzymatic)
£234.00 (exc VAT) per 5G
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