Bluo-Gal, reagent for selection of recombinant bacterial clones
Application
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.
Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as ?-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
General description
Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Packaging
100, 500 mg in poly bottle
Principle
When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
| Quality Level | 200 |
|---|---|
| InChI key | LINMATFDVHBYOS-MBJXGIAVSA-N |
| InChI | 1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1 |
| Manufacturer | SIGMA-ALDRICH |
| Solubility | DMF: soluble |
| Form | powder |
| Assay | ≥98% (HPLC) |
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