Ubiquitin Activating Enzyme (E1) is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner.
Other Notes
Manufactured for Sigma by Boston Biochem. Inc.
Physical form
Solution in 50 mM HEPES, pH 8.0, 50 mM NaCl and 1mM TCEP