BMP-4 human,;, recombinant, expressed in E. coli, >=98% (SDS-PAGE), >=98% (HPLC), cell culture teste

Stock Code: 3592994
Manufacturer Part No: SRP3016-10UG

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Descriptions

Application

BMP-4 human has been used for in vitro chondrogenesis in goat adipose tissue-derived mesenchymal stem cells.

Biochem/physiol Actions

Members of TGF-? (transforming growth factor ?) superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. Like BMP-7, BMP-4 (bone morphogenetic protein 4) is involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. BMP-4 controls the expression of miRNAs in breast cancer cell lines, thereby regulating the proliferation and migration phenotypes. It is involved in carcinogenesis and exhibits its effect through the SMAD-dependent signaling. In mice, homozygous mutations in BMP4 cause early embryonic lethality.

General description

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-? (transforming growth factor ?) superfamily of structurally related signaling proteins. Escherichia coli derived BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits which correspond to amino acids 303-408 of the full length BMP-4 precursor.

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening.? Reconstitute in 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml.? Do not vortex.? This solution can be stored at 2-8° C for up to 1 week.? For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20° C to -80° C.

Sequence

KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR