Pyruvate Dehydrogenase Phosphatase from bovine kidney, buffered aqueous glycerol solution, approx. 8

Pyruvate Dehydrogenase Phosphatase from bovine kidney, buffered aqueous glycerol solution, approx. 8

Stock Code: 3591983
Manufacturer Part No: P2743-1VL
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Biochem/physiol Actions

Insulin-activated Mg2+-dependent and Ca2+-stimulated mitochondrial protein phosphatase specific for the pyruvate dehydrogenase complex.

Pyruvate dehydrogenase phosphatase catalytic subunit 1 (PDP1) catalyzes a Mg2+,-Ca2+ mediated dephosphorylation and activation of E1. PDP1 plays a crucial role in the regulation of pyruvate dehydrogenase complex (PDC) activity.

General description

Pyruvate dehydrogenase phosphatase (PDP) (EC 3.1.3.43) contains two genetically and biochemically different isoforms, PDP1 and PDP2. PDP1 is widely distributed and is composed of two subunits PDP1c, a catalytic subunit and PDP1r, a flavoprotein with a bound FAD.

Physical form

Solution in 50 µl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, and 50% glycerol.

Unit Definition

One unit will releases 1 nmol of inorganic phosphate from 32P-labeled pyruvate dehydrogenase complex per min at 30 °C, pH 7.0.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−70°C
Formbuffered aqueous glycerol solution

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