Phospholipase D from Streptomyces chromofuscus, >=50,000 units/mL, buffered aqueous glycerol solutio

Phospholipase D from Streptomyces chromofuscus, >=50,000 units/mL, buffered aqueous glycerol solutio

Stock Code: 3591928
Manufacturer Part No: P0065-25KU
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Application

Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).

Biochem/physiol Actions

Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.

Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

General description

Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.

Physical form

Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).

Unit Definition

One unit will release 1 µmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−70°C
Formbuffered aqueous glycerol solution

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