alpha-Lactalbumin from bovine milk, For use as a marker in SDS-PAGE

Stock Code: 3591664
Manufacturer Part No: L6385-1VL

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Descriptions

Application

?-Lactalbumin from bovine milk is suitable for use:as an electrophoresis marker, with a molar mass of approximately 14,200Dain a study to investigate selective binding of proteins on charged surface iron oxide nanoparticles via reverse charge parity model?-Lactalbumin was used in the reduction of the antigenicity of whey proteins by lactic acid fermentation.

Biochem/physiol Actions

Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp⁸⁷ or Asp⁸⁸ to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to ﹤3.5% of the maximal rate.

?-Lactalbumin consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. ?-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native ?-lactalbumin from the reduced, denatured form. ?-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Angstrom resolution, demonstrating four a-helices and a triple stranded antiparallel ?-sheet.

General description

?-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.