Bovine Serum Albumin, heat shock fraction, protease free, low endotoxin, suitable for cell culture,

Stock Code: 3589547
Manufacturer Part No: A4919-100G

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Descriptions

Application

Select the right albumin for your application with the Albumin Selection Guide

Bovine Serum Albumin has been used:as a component of cell culture media in cochlear explant culture organotypic cultures and embryo culture as a component of phosphate-buffered saline (PBS)in immunostaining of human induced pluripotent stem cell (hiPSC)

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most vitalconstituent of the cellculture media, which aidsembryonic stem cells (hESC) differentiation.It acts as a blocking agent in enzyme linked immunosorbent assay (ELISA) to inhibit non-specific binding of antigens and antibodies to the microtiter plates.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

Tested for use in cell culture Protease-free Low endotoxin

General description

Bovine serum albumin (BSA), a 66kDa protein, belongs to the family of serum albumins. It is an ?-helical, non-glycosylatedand globular protein,that has 17-disulfidebonds.

Packaging

1, 5, 25, 100 g in poly bottle

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.