N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide has been used as a chromogenic substrates for determining caspase-3 and caspase-8 activities in liver tissue homogenates and caspase 3 activity in aortic vascular smooth muscle cells and neuroblastoma cell lines.
Biochem/physiol Actions
Colorimetric substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase.