Senescence Detection Kit

Stock Code: 3587300
Manufacturer Part No: QIA117-1KIT
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Components


Fixative Solution, X-Gal, Staining Solution, Staining Supplement, and a user protocol.


General description


Useful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. Higher levels of β-Galactosidase is considered as a good marker for senescent cells. The mechanism of this is believed to be due to the increased lysosomal content of senescent cells, resulting in an increase of the lysosomal enzyme β-galactosidase. Cells can be observed using microscope.


Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. It represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or by passage in culture. Senescent cells display an increase in cell size, pH-dependent expression of β-galactosidase (β-Gal) activity, and altered patterns of gene expression.


Legal Information


CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany


Other Notes


Kurz, D.J., et al. 2000. J. Cell Sci.113, 3613.Dimri, G.P., et al. 1995. Proc. Natl. Acad. Sci. USA92, 9363.


Packaging


1 kit in Glass bottle


Preparation Note


PBS: Prepare a 1X PBS solution (not provided). Use at least 3 ml per well. X-gal: Dissolve 20 mg X-gal in 1 ml of DMF (N-N-dimethylformamide, not provided) to prepare a 20X stock solution. Aliquot and freeze (-20°) the unused X-gal solution. This stock solution is stable for up to 1 month at -20°C (protect from light). Always store X-gal in a polypropylene or glass container. Do not use polystyrene. Staining Solution Mix: While cells are incubating in Fixative Solution (step 2 below), prepare Staining Solution Mix in a polypropylene plastic tube by adding 470 µl of Staining Solution, 5 µl Staining Supplement, and 25 µl of 20 mg/ml X-gal in DMF.


Principle


The Calbiochem® Senescence Detection Kit is useful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. The mechanism of this is believed to be the increased lysosomal content of senescent cells resulting in an increase of the lysosomal enzyme β-galactosidase.


Specifications


Assay Time: 30 min. plus overnight incubation


Storage and Stability


Upon arrival store the entire contents of the kit at -20°C.


Warning


Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Quality Level100
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C

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