Lectin from Phytolacca americana (pokeweed), lyophilized powder, BioReagent, suitable for cell cultu

Lectin from Phytolacca americana (pokeweed), lyophilized powder, BioReagent, suitable for cell cultu

Stock Code: 3587112
Manufacturer Part No: L8777-10MG
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Analysis Note


Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.


Application


Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).


Biochem/physiol Actions


PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.


Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.


General description


Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.


Packaging


5, 10 mg in glass bottle


Quality


Partially purified TCA precipitate.Essentially salt-free.

Quality Level200
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Product LineBioReagent
Storage Temp.2-8°C
SolubilityPBS: 1 mg/mL
Formlyophilized powder

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