In Vitro Osteogenesis AssayKit

Stock Code: 3586917
Manufacturer Part No: ECM810

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Descriptions

Application

Note: Kit componenet 10X ARS Dilution Buffer: Part No. 2004810One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent is provided. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use.

Research CategoryCell Structure

This In Vitro Osteogenesis Assay Kit provides the necessary reagents & protocols for efficient in vitro differentiation of MC3T3-E1 cells to mature osteoblasts & for the end-point determination & quantification of this process.

Components

Ascorbic Acid 2-Phosphate Solution (500X): Part No. 2004011 One vial containing 500 µL of 100 mM ascorbic acid 2-phosphate in deionized water. Store at -20°C.Glycerol 2- Phosphate Solution (100X): Part No. 2004010 Three vials containing 1 mL of 1 M glycerol 2-phosphate in deionized water. Store at -20°C.Melatonin Solution: Part No. 2004353 One vial containing 6 µg of melatonin. Reconstitute before use with 500 µL distilled H₂O for a final concentration of 50 µM. Store at -20°C.Alizarin Red S Stain Solution (1X): Part No. 2003999 One bottle containing 50 mL Alizarin Red S Solution. Store at 2°-8°C.10% Acetic Acid: Part No. 2004807 One vial containing 20 mL of 10% Acetic Acid in deionized water. Store at 2°-8°C.10% Ammonium Hydroxide: Part No. 2004809 One vial containing 10 mL of 10% Ammonium Hydroxide in deionized water. Store at 2°-8°C.10X ARS Dilution Buffer: Part No. 2004810 One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use. Store at 2°-8°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

Bone matrix is a dense mixture comprised mainly of collagen fibers and calcium phosphate particles, with a population of living cells contained within. Despite its rigidity, bone continually undergoes a remodeling process that requires the coordinated activity of two types of cells, osteoclasts and osteoblasts. Many diseases of bone including osteoporosis, a common phenomenon in post-menopausal women in which bone mass is greatly reduced, and osteogenesis imperfecta, also known as brittle-bone disease, are likely caused by the misregulation of osteoblasts and osteoclasts. Understanding the molecular mechanisms that underlie osteogenesis, the process by which new bone is formed, is thus of significant clinical importance.

Osteoclasts are related to macrophages, and erode the bone matrix by secreting acids and hydrolases to dissolve bone minerals and digest organic components. Osteoblasts arise from undifferentiated precursor cells, and deposit a mineralized matrix consisting of collagen, calcium, and phosphorous and other minerals, leading to the formation of new bone. Osteoblasts have also been shown to express factors which regulate the differentiation and function of osteoclasts [2]. The balance between the activity of osteoblasts and osteoclasts determines the mass and density of bone [1].