QCM Tumor Cell Transendothelial Migratio

Stock Code: 3586899
Manufacturer Part No: ECM558

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Descriptions

Application

Research CategoryApoptosis & Cancer

Calculation of ResultsResults of the assay may be illustrated graphically using a bar chart to display optical density (OD) values at ~570 nm.A typical tumor cell transendothelial migration experiment will compare with a negative control. Negative control chambers, containing HUVECs only, function to determine the level of HUVECs migrating through the membrane. Cell migration in these chambers is generally low as there is no ECM on the lower side of the membrane to support endothelial migration, and these chambers are typically used as "blanks" for interpretation of data. As such, migration in test wells can be described as the value of tumor transendothelial migration minus the amount of migration visualized in the HUVEC only control. Additional migration may also be induced or inhibited in test wells through the addition of cytokines or other pharmacological agents. The following figures demonstrate typical migration results. One should use the data at left for reference only. This data should not be used to interpret actual assay results.

This QCM Tumor Cell Transendothelial Cell Migration Assay -Colorimetric provides an efficient model to analyze the ability of tumor cells to invade the endothelium.

Components

1. Migration Test Plate: (Part No. CS202627) Two 24-well culture plates, each containing 12 human fibronectin-coated cell culture inserts (8 µm pore size), sufficient for the evaluation of 24 test samples 2. TNFα: (Part No. 2004167) One tube - 20 µL at 0.1 mg/mL.3. Cell Stain Solution*: (Part No. 20294) One vial - 10 mL 4. Extraction Buffer: (Part No. 20295) One vial - 10 mL5. 24 well Stain Extraction Plate: (Part No. 2005871) One plate.6. 96 well Stain Quantitation Plate: (Part No. 2005870) One plate.7. Swabs: (Part No. 10202) 50 each.8. Forceps: (Part No. 10203) 1 pair.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

A quantitative assay for tumor transendothelial migration has been described using a modified Boyden chamber system, (Okada, 1994; Li, 1999; Laferriere, 2001). The Boyden Chamber system is a two-chamber system with a porous membrane providing an interface between these two chambers. Endothelial cells are cultured on top of the porous membrane that is coated with an extracelluar matrix (ECM) protein. A tumor cell suspension is added above the endothelial monolayer. The invasion of tumor cells across the endothelium is determined by measuring the number of cells that migrate to the lower chamber.

Millipore′s QCM Tumor Cell Transendothelial Cell Migration Assay - Colorimetric provides an efficient model to analyze the ability of tumor cells to invade the endothelium. The assay is designed with an 8 µm pore size cell culture insert, appropriate for most cancer cell lines. The upper side of the cell culture insert is coated with fibronectin to support the optimal attachment and growth of endothelial cells. The assay allows investigators to compare the invasiveness of a variety of tumor cell lines, and to evaluate the effects of various factors influencing the process