QCM CHEMOTAXIS (8UM) CELL MIG. ASY

Stock Code: 3586883
Manufacturer Part No: ECM510

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Descriptions

Application

The QCM 8 uM 96-well Migration Assay utilizes a 8 um pore size, which is appropriate for leukocyte migration.

Research CategoryCell Structure

Components

Sterile 96-well Cell Migration Plate Assembly: (Part No. 90128) One 96-well feeder tray, and one 96-well Cell Migration Chamber plate. Area = 0.3 cm², 50,000 cells per well96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.CyQuant GR Dye™: (Part No. 90132) One vial - 75 µL

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Also available: Cell Comb^™ Scratch Assay! Get biochemical data from a scratch assay!Click HereIntroductionCell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the top side of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable. The Chemicon QCM﹤TMSYMBOL>﹤/TMSYMBOL> 96-well Migration Assay does not require cell labeling, scraping, washing or counting. The 96-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples. In the Chemicon QCM﹤TMSYMBOL>﹤/TMSYMBOL> 96-well Migration Assay, migratory cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQuant GR dye (Molecular Probes). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids. The Chemicon QCM﹤TMSYMBOL>﹤/TMSYMBOL> 96-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells. The Chemicon QCM﹤TMSYMBOL>﹤/TMSYMBOL> 96-well Migration Assay utilizes an 8 µm pore size, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments