beta-N-Acetylglucosaminidase from Streptococcus pneumoniae, recombinant, expressed in E. coli, buffe

beta-N-Acetylglucosaminidase from Streptococcus pneumoniae, recombinant, expressed in E. coli, buffe

Stock Code: 3586573
Manufacturer Part No: A6805-1VL
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Application


?-N-Acetylglucosaminidase from Streptococcus pneumoniae has been used in the removal of O-linked sugars from inositol 1,4,5-trisphosphate (InsP3) receptor type I. It has also been used in coating PVDF strips for amelogenin trityrosyl motif peptide (ATMP) and keratin 5 binding studies.


Biochem/physiol Actions


?-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the ?1-3 and ?1-6 linkages present in mucins. It belongs to the glycoside hydrolase family 85 (GH85). Also called endo-?-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides and acts on fucosylated N-glycan core. Its deglycosylation functionality is exploited in studying functions of glycoproteins.


This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.


Specific for GlcNAc; not significantly active with GalNAc


Physical form


Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl


Unit Definition


One unit will hydrolyze 1.0 µmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 5 at 37 °C.

Quality Level100
ManufacturerSIGMA-ALDRICH
Formbuffered aqueous solution

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