Lipid Hydroperoxide (LPO) Assay Kit

Stock Code: 3586502
Manufacturer Part No: 437639-1KIT

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Descriptions

Analysis Note

Positive Control13-hydroperoxyoctadecanoic acid

7. Calculating the Results:a. Calculate the average absorbance for each Lipid Hydroperoxide Standard and sample. b. Subtract the average absorbance of Lipid Hydroperoxide Standard A from itself and all other Lipid Hydroperoxide Standards and samples.c. Plot the corrected absorbance of Lipid Hydroperoxide Standards (from step b above) as a function of final hydroperoxide value from the table (see Reagent Preparation, Lipid Hydroperoxide Standards above). See below for typical standard curves using the spectrophotometric method (figure 2) and the Glass 96-Well Plate method (figure 3).﹤div class="Bio_doc_image">Figure 2: Typical standard curve using the spectrophotometric method.﹤/div>﹤div class="Bio_doc_image">Figure 3: Typical standard curve using the Glass 96-Well Plate method.﹤/div>d. Calculate the hydroperoxide values of the sample tubes (HPST) using the equation obtained from the linear regression of the standard curve substituting corrected absorbance values for each sample. HPST (nmol) = (sample absorbance - y-intercept)/slopee. Calculate the concentration of hydroperoxide in the original sample as shown below:﹤div class="Bio_doc_image">Figure 4: Calculate the concentration of hydroperoxide﹤/div>

Components

FTS Reagent 1, FTS Reagent 2, Lipid Hydroperoxide Standard, Extract R, Triphenylphosphine, and a user protocol.

General description

A sensitive and reliable assay kit for the measurement of the hydroperoxides from any sample containing lipid hydroperoxides, directly utilizing the redox reactions with ferrous ions. Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

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