ProteoExtract Native Cytoskeleton Enric

Stock Code: 3586355
Manufacturer Part No: 17-10210

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Descriptions

Application

The ProteoExtract^® Native Cytoskeleton Enrichment and Staining Kit provides cytoskeleton purification detergent buffers that retain focal adhesion and actin-associated proteins while removing soluble cytoplasmic and nuclear proteins from the cell. Anti-Vinculin and anti-GAPDH antibodies are provided as markers for focal adhesions and cytosol, respectively, in immunofluorescence analysis. This kit will greatly increase the ability to detect and study the low abundance actin-associated proteins which are typically masked in conventional methods of staining whole cells.

Research Sub CategoryCytoskeleton

The ProteoExtract Native Cytoskeleton Enrichment & Staining Kit provides cytoskeleton purification detergent buffers that retain focal adhesion & actin-associated proteins while removing soluble cytoplasmic & nuclear proteins from the cell.

Research CategoryCell Structure

Components

One bottle containing 1.9 mL of 10x Cellular Extraction BufferOne bottle containing 3.5 mL of 20x Cytoskeleton Wash BufferOne bottle containing 9 mL of ready to use Nuclear Extraction BufferOne vial containing 100 µL of 1000x Protease Inhibitor Cocktail solutionOne vial containing 150 µL of 1000x Sodium Orthovanadate solutionOne vial containing 50 µL of 200x donkey anti-mouse FITC conjugateOne bottle containing 12.5 mL of 10x Blocking/Permeabilization BufferOne vial containing 15 µg lyophilized TRITC-Conjugated PhalloidinOne vial containing 100 µL of 200x DAPIOne vial containing 100 µL of 100x anti-Vinculin mouse monoclonal IgG1 antibodyOne vial containing 16 µL of 500x anti-GAPDH mouse monoclonal IgG1 antibody

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Read our application note in Nature Methods!http://www.nature.com/app_notes/nmeth/2012/121007/pdf/an8624.pdf(Click Here!)Rediscovering the Actin Cytoskeleton: New techniques leading to discoveries in cell migration, Dr. Richard Klemke, Morris Cancer Center, UCSD, USA.The actin cytoskeleton is a highly dynamic network composed of actin polymers and a large variety of associated proteins. The functions of the actin cytoskeleton is to mediate a variety of essential biological functions in all eukaryotic cells, including intra- and extra-cellular movement and structuralSupport (Chen, C.S., et al., 2003; Frixione E., 2000). To perform these functions, the organization of the actin cytoskeleton must be tightly regulated both temporally and spatially. Many proteins associated with the actin cytoskeleton are thus likely targets of signaling pathways controlling actin assembly. Actin cytoskeleton assembly is regulated at multiple levels, including the organization of actin monomers (G-actin) into actin polymers and the superorganization of actin polymers into a filamentous network (F-actin – the major constituent of microfilaments) (Bretscher, A., et al., 1994)