To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Features and Benefits
Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use. Flexible format, protocol for classical elution as well as cross-linking method. Formatted for elution for electrophoresis or LC-MS analysis workflows. Fast binding kinetics and high capacity provides high yield. Easy scale-up with HiTrap® Protein G HP prepacked columns. Prepacked with Protein G Sepharose® High Performance for coupling of antibodies of IgG subclasses. MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.
General description
Protein G Sepharose® HP has high affinity for the Fc region of IgG from a variety of species.Protein G Sepharose® HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).A protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min is also provided. No pretreatment of the sample is required.