GSTrap Fast Flow, Cytiva, 17-5131-01, pack of 1 x 5 mL

Stock Code: 2796250
Manufacturer Part No: GE17-5131-01
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Analysis Note


To view the Certificate of Analysis for this product, please visit www.cytiva.com.


Application


GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose® 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC System.


Features and Benefits


Fast and simple one-step purification of GST-tagged Proteins. Mild elution conditions preserving Protein antigenicity and function. Easy scale-up by connecting the columns in series. Sepharose® 4 Fast FLow provides good fLow properties.


General description


GSTrap FF columns are prepacked Glutathione Sepharose® Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.


Legal Information


Sepharose is a registered trademark of Cytiva


GSTrap is a trademark of Cytiva


ÄKTA is a registered trademark of Cytiva


FPLC is a trademark of Cytiva

Capacity>10 mg/mL binding capacity(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium
Matrixhighly cross-linked 4% agarose

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