HP RNA TISSUE KIT

HP RNA TISSUE KIT

Stock Code: 2795764
Manufacturer Part No: 12033674001
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£295.29 (exc VAT) per EACH
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Application


High Pure RNA Tissue Kit has been used:to extract total RNA from human cells for cDNA library generationto extract total RNA from cultured cells for real time PCR analysisto extract total RNAs from rat hippocampito purify total RNA from cultured cells and human spinal cordto isolate total RNA of tissues collected from ventricle wallsThe isolated RNA can be used in many downstream applications:,Northern blottingRACEprimer extensiondifferential displayRNase protection assayin vitro translation


Components


Lysis/Binding Buffer DNase I, lyophilizate DNase Incubation Buffer Wash Buffer I Wash Buffer II Elution Buffer High Pure Spin Filter Tubes (containing glass fiber fleece) Collection Tubes


Features and Benefits


Prepare RNA samples in approximately 30 minutes. Obtain concentrated RNA that is suitable for downstream applications. Minimize RNA loss with a kit that removes contaminants without precipitation or other handling steps that degrade RNA. Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.


General description


The High Pure RNA Tissue Kit is designed for the purification of total, intact RNA from tissue samples, free of any contaminating DNA. Low to medium throughput RNA isolation. Nucleic acids bind to the surface of the glass fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure Filter Tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. For RNA isolation, the binding conditions can be optimized to ensure immobilization of all the RNA. High pure RNA isolation kit can rapidly isolate intact total RNA from a broad range of research sample materials, including cultured cells, mammalian blood, white blood cells (WBCs), yeast, and bacteria. It rapidly isolates total RNA from mammalian tissues such as mouse liver, spleen, lung, and heart.Capacity: The High Pure Spin Filter Tubes hold up to 700 µL sample volume.Sample Material: Solid tissue (e.g., mouse liver, spleen, lung, heart): 1 - 25 mg.Note: Sample size depends on the method used to prepare the tissue; larger samples (>10 mg) should be processed via rotor-stator homogenization.


Other Notes


Figure 1: Comparison of different homogenization procedures. Mouse liver was homogenized using various procedures. Total RNA was purified from each of these homogenates with the High Pure RNA Tissue Kit. Aliquots of the isolated RNA were reverse transcribed with primers specific for a region of the GADH mRNA. cDNA products were analyzed via gel electrophoresis.Lane 1: Homogenization: Ultra Turrax; yield: 1.9 µg/mg tissue; A 260/ A 280 : 2.0Lane 2: Homogenization: motor-driven, disposable plastic pestle; yield: 3 µg/mg tissue; A 260 /A 280 : 2.0Lane 3: Homogenization: mortar and pestle, 20 G needle; yield: 1.5 µg/mg tissue; A 260 /A 280 : 2.0Lane 4: Homogenization: manual disposable plastic pestle, 20 G needle; yield: 1.8 µg/mg tissue; A 260 /A 280 : 2.0Lane 5: Homogenization: manual disposable plastic pestle; yield: 3

Quality Level100
Technique(s)RT-PCR: suitable, Northern blotting: suitable

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