DNA ISOLATION KIT FOR MAMMALIAN BLOOD

DNA ISOLATION KIT FOR MAMMALIAN BLOOD

Stock Code: 2795677
Manufacturer Part No: 11667327001
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Application


DNA Isolation Kit for Mammalian Blood has been used in isolation of genomic DNA from blood of asthmatic patients for single nucleotide polymorphisms genotyping.


The DNA Isolation Kit for Mammalian Blood purifies DNA that is suitable for most molecular biology applications: PCR/long PCR Sequencing Restriction digestion Southern blotting Cloning


Components


Red Blood Cell Lysis Buffer White Blood Cell Lysis Buffer Protein Precipitation Solution


Features and Benefits


Process multiple samples simultaneously.DNA is easily purified in ﹤90 minutes (plus 30 to 60 minutes resuspension time). Use a cost-effective kit.Achieve faster DNA purification for the cost of most homebrew methods. Obtain consistent and reliable results.Analyze different sample volumes (1 to 10 mL) with varying amounts of leukocytes. Improve safety.Eliminate the use of chaotropic salts, hazardous organic solvents, and column purification steps.


General description


Isolation of DNA from whole blood can be difficult because blood is a complex mixture containing cells, proteins, metabolites, etc. Most of the cells (>99%) are erythrocytes (red blood cells) which lack nuclei, and therefore, possess no DNA. Leukocytes (white blood cells) contain nuclei and DNA. Therefore, DNA from blood must be isolated from one of three types of leukocytes: monocytes, lymphocytes, or granulocytes.Sample material: Human whole blood (treated with sodium heparin, EDTA or sodium citrate): 1 to 10 mLIsolated lymphocytes: 1 to 10 mLIsolated buffy coat: buffy coat from 10 to 20 mL whole blood, containing at least 1.0 x 107 leukocytes.Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9


The DNA Isolation Kit for Mammalian Blood rapidly isolates DNA from 1 to 10 ml mammalian whole blood, lymphocytes, or buffy coat samples.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Figure 1: Amplification of large DNA fragments from mammalian blood prepared with the DNA Isolation Kit for Mammalian Blood. DNA was prepared from two research samples, one containing 10 ml human blood, the other containing 10 ml mouse blood. Aliquots of each DNA preparation were used as templates for the amplification of several gene fragments with the Expand Long Template PCR System. PCR products were analyzed by gel electrophoresis.Left panel: Gene fragments amplified from human samplesLanes 2, 3: tPA fragment (9.3 kb) amplified from 25 ng DNALanes 4, 5: tPA fragment (15 kb) amplified from 50 ng DNALanes 6, 7: β-globin fragment (23 kb) amplified from 100 ng DNALanes 8, 9: β-globin fragment (28 kb) amplified from 200 ng DNALanes 1, 10: DNA Molecular Weight Marker IIIRight panel: Gene fragments amplified from mouse samplesLanes 2, 3: IL-2 gene (4.2 kb) amplified from 330 ng DNALanes 4, 5: α-2 collagen fragment (5.6 kb) amplified from 100 ng DNALanes 6, 7: α-2 collagen fragment (10.4 kb) amplified from 50 ng DNALanes 8, 9: α-2 collagen fragment (15.4 kb) amplified from 100 ng DNALanes 1, 10: DNA Molecular Weight Marker III


Figure 2: Southern blot analysis of DNA from various human blood samples prepared with the DNA Isolation Kit for Mammalian Blood

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