ROCHE Chymotrypsin Sequencing Gradefrom bovine pancreas

ROCHE Chymotrypsin Sequencing Gradefrom bovine pancreas

Stock Code: 2795601
Manufacturer Part No: 11418467001
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£176.89 (exc VAT) per EACH
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Application


Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.


General description


Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Preparation Note


Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.Storage conditions (working solution): 2 to 8 °CA solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.


Quality


Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.


Reconstitution


Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl2, pH 7.8).


Specificity


Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Quality Level100
Formlyophilized (salt-free)
Assay≥90%

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