Interlueken 2 Mouse Mil-2

Stock Code: 2795568
Manufacturer Part No: 11271164001

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Descriptions

Application

Recombinant murine IL-2 is produced in E. coli and exhibits the following:Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells.Strongly inhibits the binding of recombinant human IL-2 to murine responder cells.Weakly inhibits the binding to human responder cells.Shares identical biological and immunological activities with human IL-2.Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models.used for the stimulation of murine T-cells

General description

Recombinant Interleukin-2, mouse (mIL-2), is produced in E. coli and purified by standard chromatographic methods.

Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 µm pore size membrane

Interleukin-2 (IL-2, also known as T-cell growth factor, TCGF) is a lymphokine produced by lectin- or antigen-activated T-cells. It plays an important immunoregulatory role. This factor was first identified by its ability to promote the long-term in vitro proliferation of activated T-cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells. IL-2 also induces other lymphokines such as interferon-γ and B-cell growth factor (BCGF-1).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Specific activity/EC ₅₀: >2 x 10⁶ U/mg, ﹤0.5 ng/ml (hIL-2, NIBSC, 1^st international standard, 86/504), at least the same specific activity (EC₅₀) compared to the indicated standard is guaranteed.Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.EC ₅₀ definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).

Preparation Note

Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v).Storage conditions (working solution): -15 to -25 °CNote: Avoid repeated freezing and thawing.

Quality

Endotoxin level: ﹤0.1 EU/µg (LAL-test), ﹤10 EU/ml (LAL-test)

Sequence

Chain Length 149 AA

One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity.

Specificity

Mouse IL-2 is effective on mouse cells, but not on human cells.

Unit Definition

EC₅₀ definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).