ROCHE ABTS Buffer

ROCHE ABTS Buffer

Stock Code: 2795554
Manufacturer Part No: 11204530001
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Application


ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.


General description


2,2?-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.


Legal Information


ABTS is a trademark of Roche


Other Notes


For life science research only. Not for use in diagnostic procedures.


Preparation Note


Working concentration: Flow cytometry: 0.5 µg/100 µl (106 cells); Immunohistocytochemistry: 50 µg/mlWorking concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be ﹤0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

Quality Level100
Storage Temp.2-8°C
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