ROCHE Glutamate-Pyruvate Transaminase (GPT)from pig heart

Stock Code: 2795453
Manufacturer Part No: 10737127001

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Descriptions

Application

Synthesis of unnatural L-amino acids from α-keto acids.

General description

Glutamate-pyruvate transaminase is a blood enzyme. It metabolizes glutamate in blood resulting in low extracellular levels of brain glutamate.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: PyridoxalphosphateStorage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution.

Quality

Contaminants: ﹤ 0.01% GIDH, LDH, and MDH each, ﹤ 0.03% GOT

Unit Definition

Unit Conversion: 1 U [+25 °C, pH 7.5] ≈ 1.75 U [+37 °C, pH 7.5]