ROCHE n-Octylglucosidenon-ionic

ROCHE n-Octylglucosidenon-ionic

Stock Code: 2795435
Manufacturer Part No: 10634425001
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Application


N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It is a mild and non-denaturing detergent for the solubilization and reconstitution of membrane proteins. N-Octylglucoside is easily removed by dialysis. N-Octylglucoside has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.


Non-ionic, dialyzable detergent for the solubilization and isolation of membrane proteins. Has been shown to increase the resolution of proteins in 2D gels.


General description


1-O-n-Octyl-?-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Preparation Note


Working concentration: 46 mMWorking solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.Storage conditions (working solution): Stability in SolutionA stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.


Quality


Purity: 99% (from C), homogeneous (GC) Ease of removal*: ++CMC**: 14.5 mM at +25°CFormula: C14H28O6


Storage and Stability


Store at 15 to 25 °C. (Store dry!)

InChI keyHEGSGKPQLMEBJL-RKQHYHRCSA-N
InChI1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1
Assay99% (GC)

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