Nadh Approx 98% 1g

Stock Code: 2795402
Manufacturer Part No: 10128023001
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Application


NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.


NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay.


As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.


Biochem/physiol Actions


NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Packaging


Packaged by solid weight.


Physical form


Amorphous powder


Preparation Note


Activator: Mg2+ (5 to 10 mM)Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C


Quality


Purity: 98% β-NADH-Na2 based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A260 nm-A340 nm: ﹤2.40Organic solvents: ﹤4% (GC)


Specifications


Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at 334 nm (Hg), 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at 365 nm (Hg)Formula: C21H27N7O14P2Na2pH value in water: 7.5 ± 0.5 (100 mg/ml)


Storage and Stability


Store at 2 to 8 °C. (Store dry!)

InChI keyFWBNCIFELNNJCX-UHOVGGJYSA-L
InChI1S/C21H29N7O14P2.2Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(2
Formamorphous powder
Assay98%

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