cOmpleteMiniEASYpack30 Tab.

Stock Code: 2795267
Manufacturer Part No: 4693124001

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Descriptions

Application

cOmplete^™, Mini, is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.cOmplete, Mini, contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete, Mini, to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps.cOmplete, Mini, contains EDTA (1 tablet yields a 1 mM EDTA solution in 10 ml). Therefore, the extraction buffer should not contain divalent cations like Ca²⁺, Mg²⁺, or Mn²⁺, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography.Alternatively, the product cOmplete, Mini, EDTA-free, can be used. These tablets are identical to cOmplete, Mini, with the exception that no EDTA or other chelating agent is present.cOmplete, Mini Tablets, inhibit proteolytic activity in small extraction volumes (up to 10 ml). If you are working with a larger volume (up to 50 ml), we recommend to use a different cOmplete product. The composition of the cOmplete Tablet is identical to the cOmplete, Mini Tablet, therefore, comparable results are achieved with both product types.

cOmplete^™, Mini Protease Inhibitor Cocktail has been used as a component in mammalian-protein extraction reagent (M-PER) for lysis of glioma stem cells (GSCs) sodium dodecyl sulphate-tris buffered saline (SDS-TBS) for the homogenisation of brain tissuesradioimmunoprecipitation assay (RIPA) buffer for lysis of human embryonic stem cells (hESCs)

Components

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Features and Benefits

Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer. Complete protection: Instantly protect your proteins against a broad range of proteases (see Table 1). Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi. Safe: Choose non-toxic inhibitors that pose no risk to you or those around you. Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.

General description

cOmplete, Mini Protease Inhibitor Tablets, inhibit a broad spectrum of serine, cysteine and metalloproteases, as well as calpains. Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.cOmplete, Mini, contains EDTA and both irreversible and reversible protease inhibitors.

Legal Information

cOmplete is a trademark of Roche

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

30 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters. Each tablet is sufficient for a volume of 10 ml solution.

Physical form