Tris-EDTA buffer solution, 100 x, for molecular biology

Tris-EDTA buffer solution, 100 x, for molecular biology

Stock Code: 3577475
Manufacturer Part No: T9285-100ML
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Application


Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks.Tris-EDTA buffer solution has been used in culture medium.Tris-EDTA buffer solution has been used for the preparation of AmpC disks for permeabilization of Tris-EDTA to release ?-lactamases from bacterial cell.It has been used for the suspension of bacterial spores.It has also been used as a buffer in immunohistochemistry.


Components


TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.


General description


TE buffer is the most commonly used buffer solution in molecular biology. "TE" is a component of Tris (a common pH buffer) and EDTA (molecule that chelate cations). TE (Tris EDTA) Buffer solubilizes DNA and RNA while protecting it from degradation. TE Buffer is used in nucleic acid isolation, which may be done prior to Northern or Southern blot hybridization.

Quality Level200
Pack100ML
pH7.8-8.2 (1 × in water)
InChI keyVLEIUWBSEKKKFX-UHFFFAOYSA-N
InChI1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
ManufacturerSIGMA-ALDRICH
Formliquid

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