MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers
Application
Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.
Components
1x MESA buffer contains: 40 mM MOPS 10 mM sodium acetate 1 mM EDTA (pH 8.3)
General description
MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.
Packaging
1 L in poly bottle
Reconstitution
Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.
| Quality Level | 200 |
|---|---|
| Pack | 1L |
| pH | 6.8-7.2(reconstituted per directions) |
| Manufacturer | SIGMA-ALDRICH |
| Product Line | BioReagent |
| Storage Temp. | room temp |
| Solubility | H2O: soluble, faintly yellow |
| Form | powder |
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