Tris Acetate-EDTA buffer, BioReagent, suitable for electrophoresis

Tris Acetate-EDTA buffer, BioReagent, suitable for electrophoresis

Stock Code: 3577348
Manufacturer Part No: T6025-1L
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Application


TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.


Tris Acetate-EDTA buffer has been used as run buffer for the agarose gel electrophoresis of human papillomavirus DNA and transcribedRNA samples.


Other Notes


40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.


Preparation Note


Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).


Solution prepared with 18 megohm water

Quality Level200
Pack1L
InChI keyHGEVZDLYZYVYHD-UHFFFAOYSA-N
InChI1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
ImpuritiesDNase, RNase, Protease, none detected
Formworking solution

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