Tetanus toxin C fragment from Clostridium tetani,

Stock Code: 3576972
Manufacturer Part No: T3694-10UG

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Descriptions

Application

The C terminal binding portion of tetanus toxin is not toxic but is taken up by pre-synaptic nerve terminals and exhibits trans-synaptic retrograde transport in central neurons similar to that of tetanus toxin. It is used to map neuronal connections and to target proteins to neurons.

Biochem/physiol Actions

Tetanus toxin is a neurotoxin that comprises of a heavy and a light chain linked by disulfide bridges. This neurotoxin enters the cytoplasm and releases the light chain component that subsequently inhibits the release of neurotransmitters². Studies have reported that the fragment C of tetanus toxin retains most of the determinants that interact with thyroid membranes, gangliosides and neural membranes³.

General description

Tetanus toxin C fragment (TTC) is a non-toxic peptide, which is transported from peripheral axons into spinal motoneurons. It provides protection for motoneurons (MN) in the spinal cord in vivo. TTC regulates membrane binding, internalization and retrograde transport within MN. It initiates neurotrophin-regulated signaling pathways in a Trk receptor-dependent manner. TTC has neuroprotective property in primary cerebellar granule cell culture.

Reconstitution

Each vial, when reconstituted with 50 µl of sterile distilled water, contains 10 µg of tetanus toxin C-fragment in 0.01 M sodium phosphate, pH 7.5. To aid recovery, 0.1% albumin may be added.