4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide, beta-N-acetylhexosaminidase substrate
Application
4-Methylumbelliferyl N-acetyl-?-D-glucosaminide has been used as a substrate for assaying?-N-acetylglucosaminidase (NAGase) from soil sampleschitinase from Fusarium oxysporum using fluorimetric assay?-N-acetylhexosaminidases from Arabidopsis thaliana roots and leaves samples
Biochem/physiol Actions
4-Methylumbelliferyl N-acetyl-?-D-glucosaminide (4-MUF-NAG) is a synthetic fluorogenic substrate for N-acetyl-?-D-glucosaminidase (NAGase). The enzyme functionality is assessed by its ability to hydrolyze the N-acetyl-D-glucosamine (NAG) residue in 4-MUF-NAG. 4-MUF-NAG is also a substrate for Chitinase and N-acetylhexosaminidase.
General description
4-Methylumbelliferyl N-acetyl-?-D-glucosaminide (4-MUF-NAG) contains chitin monomer, N-acetyl-D-glucosamine (NAG) and a 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF) moiety.
Packaging
1 g in poly bottle
25, 100, 250 mg in poly bottle
Substrates
Fluorogenic substrate for β-N-acetylhexosaminidase
| Quality Level | 200 |
|---|---|
| InChI key | QCTHLCFVVACBSA-XWEABGILSA-N |
| InChI | 1S/C18H21NO8/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21)/t13-,15-,16-,17-,18?/ |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Solubility | DMF: 20 mg/mL, clear, colorless to faintly yellow |
| Form | powder |
| Assay | ≥98% (TLC) |
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