2-Nitrophenyl beta-D-galactopyranoside, >=98% (enzymatic)

Stock Code: 3577219
Manufacturer Part No: N1127-500MG

Order Now for 21 day delivery

Descriptions

Application

2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.

Biochem/physiol Actions

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

General description

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Packaging

500 mg in poly bottle

250 mg in glass bottle

1, 5, 25 g in poly bottle

Principle

Reconstitution

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

Substrates

Chromogenic substrate for β-galactosidase

Specifications

Quality Level	200
InChI key	KUWPCJHYPSUOFW-YBXAARCKSA-N
InChI	1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
Manufacturer	SIGMA-ALDRICH
Storage Temp.	−20°C
Form	powder
Assay	≥98% (enzymatic)