Dulbeccos Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, powd
Application
Dulbecco?s Phosphate Buffered Saline has been used:as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloyto dissolve Angelica gigas powder for microencapsulationto wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assayto wash amniotic membrane for cellular analyses
D-PBS is used to wash cells during preparation and serial transfer.
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.
General description
Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.
Other Notes
This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available
Quantity
Formulated to contain 9.6 grams of powder per liter of medium.
| Quality Level | 500 |
|---|---|
| pH | 7.2-7.8 |
| Manufacturer | SIGMA-ALDRICH |
| Technique(s) | cell culture - mammalian: suitable |
| Storage Temp. | 2-8°C |
| Solubility | water: 9.6 g/L, clear |
| Form | powder |
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