Acrylamide/Bis-acrylamide, solution, BioReagent, suitable for electrophoresis, 29:1

Acrylamide/Bis-acrylamide, solution, BioReagent, suitable for electrophoresis, 29:1

Stock Code: 3577351
Manufacturer Part No: A3574-5X100ML
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Application


Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 4% stacking gel and 10% resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis. It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods.


These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.


General description


Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.


Preparation Note


Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Quality Level200
InChI keyOCQZXMCGTAWGEQ-UHFFFAOYSA-N
InChI1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
Formliquid

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