Deoxyribonuclease I RNase-free solution from bovine pancreas,

Descriptions

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Components

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5?phosphates and 3?OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

Other Notes

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Unit Definition

One unit will cause a δA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Form	Price		Quantity
3577270	Deoxyribonuclease I RNase-free solution from bovine pancreas,	200	SIGMA-ALDRICH	buffered aqueous glycerol solution	£266.04 (exc VAT) per EACH		- +
3577271	Deoxyribonuclease I RNase-free solution from bovine pancreas,	200	SIGMA-ALDRICH	buffered aqueous glycerol solution	£880.29 (exc VAT) per 2MG		- +