Schiffs reagent for microscopy

Stock Code: 3595667
Manufacturer Part No: 1090332500
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Application


For microscopy. Ready-to-use staining solution for human-medical cell diagnosis.


General description


Schiff‘;s reagent -for microscopy for the periodic acid Schiff, or PAS reaction, is a ready-to-use solution used for the detection of mucopolysaccharides, glycogen, muco- and glycoproteins, glyco-, phospholipids, basal membranes and collagen. The PAS (periodic acid Schiff) reaction is one of the most frequently used chemical methods for the detection of aldehyde and mucosubstances in histology. In the PAS reaction, the histological specimen material is first treated with periodic acid, resulting in the oxidation of the 1,2-glycols into aldehyde groups. The addition of Schiff’;s reagent (fuchsin-sulfuric acid) in the second step causes the aldehydes to react to form a brilliant red color. In the end result, the PAS reaction yields a specific color reaction with unsubstituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glyco- and phospholipids. The PAS reaction can be further combined with the Alcian blue staining method to detect mucosubstances (glycosaminoglycans). 500 ml are sufficient for approx. 2500 - 3000 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.


Packaging


500 ml in Glass bottle


2.5 l in Glass bottle

Quality Level400
pH2.1-2.5 (25 °C in H2O)
ManufacturerSIGMA-ALDRICH
Storage Temp.15-25°C
Density1.01 g/cm3 at 20 °C
Formliquid

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