ROCHE Xanthine Oxidase (XOD)from cow milk

ROCHE Xanthine Oxidase (XOD)from cow milk

Stock Code: 2795380
Manufacturer Part No: 10110434001
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Application


Xanthine Oxidase has been used to study tyrosine nitration.


Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.


Biochem/physiol Actions


Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.


General description


Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.


Xanthine:oxygen oxidoreductase


Other Notes


For life science research only. Not for use in diagnostic procedures.


Physical form


Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8


Preparation Note


Activator: O2Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.


Quality


Contaminants: ﹤0.005% guanase, NP and uricase, each, ﹤0.05% ADA, ﹤0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)Note: Chromatographically purified.


Sequence


XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.


Unit Definition


One unit (U) xanthine oxidase will produce 1 µmol of uric acid (E293nm = 12.2 mmol -1 x L x cm-1) from the oxidation of 1 µmol of xanthine in 1 min at 25 °C and pH8.5.

Quality Level100
Formsuspension

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