RTG GenomiPhi(TM) V3 DNA Amplification Kit; 24 RXN; Cytiva; 25-6601-24; sufficient for 24 reactions

RTG GenomiPhi(TM) V3 DNA Amplification Kit; 24 RXN; Cytiva; 25-6601-24; sufficient for 24 reactions

Stock Code: 2793426
Manufacturer Part No: GE25-6601-24
Order Now for 21 day delivery
£253.50 (exc VAT) per EACH
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Analysis Note


To view the Certificate of Analysis for this product, please visit www.cytiva.com.


Application


For whole genome amplification by isothermal strand displacement amplification; typical downstream applications are genotyping (SNP, STR, array CGH) and DNA archiving.


Features and Benefits


Representative amplification of the whole genome. Shipping and storage at room temperature provides easy access and reduced carbon footprint. Quick and simple automation-friendly protocol; no thermal cycler required. Yields high-quality DNA (e.g. for high-throughput genotyping and DNA archival). Preformulated, predispensed, single-dose, lyophilized cakes for rapid reaction set-up and greater reproducibility.


General description


illustra™ Ready-To-Go GenomiPhi™ V3 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for whole genome amplification by isothermal strand displacement amplification. The reaction mixture is provided as predispensed, single-dose, lyophilized cakes in either strips of 8 tubes, or 96-well plates.Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity. The average product length is over 10 kb.The illustra™ Ready-To-Go GenomiPhi™ V3 DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 12 to 20 µg DNA can be achieved in less than two hours from only 10 ng of genomic DNA input. The starting material for GenomiPhi™ reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual.GenomiPhi™ amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.


Legal Information


illustra is a trademark of Cytiva


GenomiPhiOn packaging and insert:For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.On container:Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.


GenomiPhi is a trademark of Cytiva

Storage Temp.room temp

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