Vivaspin sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.
Vivaspin sample concentrators cater for sample volumes from 100 µl to 20 ml, with a range of molecular weight cutoff values from Mr 3 000 to 100 000. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest.
One-step sample concentration in a single tube for minimal sample handling and reduced sample loss.
Patented dead-stop technology, which ensures that samples cannot be concentrated to dryness and enables direct concentrate recovery
Vertical polyethersulfone membrane minimises membrane blockage and tolerates high flow rates.
Compatible pH range is from pH 1 to 9.
Easy, contact-free storage by reverse spinning the concentrate into the recovery cap