The 4 spot area QIAcard FTA PlantSaver format provides a laminated flap that allows you to vigorously crush plant or insect samples into the FTA matrix without damaging the FTA card.
Features:
Simply pressing plant samples onto the FTA matrix or applying homogenate captures and stabilises the DNA.
Store and collect at room and high temperatures for convenient collection in the field.
Smaller sample size: Only young leaves are required, reducing growing time and speeding research.
Fast purification in 30 minutes: PCR-ready DNA can be purified on FTA in three simple steps, all in a single tube at room temperature.
Application:
Analyse plant DNA by PCR-based assays
Detection of Genetically Modified (GM) organisms
Marker selected breeding
Varietal identification
Phylogeny analysis
Amplification of low copy number loci
Invader(TM) assays
Multiple displacement amplification
Transgenic identification
Analysis:
Plant tissue is physically crushed on the card and the DNA binds to the matrix
The chemical coating on the FTA card protects the DNA from degradation and allows the cards to be stored at room temperature for extended periods of time. To prepare the sample for PCR, the matrix is washed with two nontoxic aqueous buffers
The DNA remains bound to the matrix throughout purification and a small disk (1.2 or 2mm in diameter) of the matrix provides enough template for PCR analysis