Qiagen QIAcard FTA Clonesaver 96-well format

Stock Code: 3246612
Manufacturer Part No: FIL5800

Descriptions

FTA technology consists of two distinct chemistries, both of which have the ability to lyse cells on contact, denature proteins, and protect DNA from degradation. FTA chemistry contains chemical denaturants and a free radical scavenger. With FTA, the DNA remains tightly bound while proteins and inhibitors are washed from the matrix. There are three options for DNA analysis with FTA cards: direct amplification, standard amplification with rinsing ("punch-in" method), and extraction of DNA from the card.

QIAcard FTA indicating cards are designed for collection, stabilisation, processing, transport, and archiving of buccal nucleic acid samples. The cards include a pink indicating dye that turns to white when a clear sample such as buccal cells or saliva is applied. Each sample area of a card can be processed separately. This versatility of handling supports manual or automated processing and incorporation into current workflows.

The QIAcard FTA CloneSaver 96 well format employs a filter-based method for archiving and purifying clones. The functions of -80C freezers and purification kits are condensed to the size of an index card. CloneSaver uses FTA technology to provide years of ambient clone archiving, with protection against and recovery from phage infection, to deliver analysis quality DNA. Clones that are stored and purified using QIAcard FTA CloneSaver are suitable for many common downstream applications including PCR and transformation (electroporation and heat shock).