NESFATIN-1 human,;, recombinant, expressed in E. coli, >=98% (SDS-PAGE), >=98% (HPLC)

NESFATIN-1 human,;, recombinant, expressed in E. coli, >=98% (SDS-PAGE), >=98% (HPLC)

Stock Code: 3593240
Manufacturer Part No: SRP3291-100UG
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Biochem/physiol Actions

Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues.

General description

Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets β-cells, gastric endocrine cells and adipocytes.? Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 6.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Colourwhite to off-white
ManufacturerSIGMA-ALDRICH
Impurities﹤0.1 EU/µg endotoxin, tested
Formlyophilized
Assay≥98% (SDS-PAGE), ≥98% (HPLC)

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