PDGF-AA from mouse,;, recombinant, expressed in E. coli, >=98% (SDS-PAGE), >=98% (HPLC), cell cultur

Stock Code: 3593184
Manufacturer Part No: SRP3228-10UG

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Descriptions

Application

PDGF-AA from mouse has been used as a supplement in the media used for differentiation of neurospheres.

Biochem/physiol Actions

The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-AA is crucial for optimal wound healing.

General description

The PDGFs are stored in platelet ?-granules and are released upon platelet activation. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-? and PDGFR-?. PDGFR-? is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-? interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids). Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids).

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sequence

MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT