SCGF-beta human,;, recombinant, expressed in E. coli, >=98% (SDS-PAGE), >=98% (HPLC), cell culture t

Stock Code: 3593118
Manufacturer Part No: SRP3153-10UG
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Biochem/physiol Actions

SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. Recombinant human SCGF-b is a 25.0 kDa polypeptide containing 227 amino acid residues.

General description

SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF-b is a 25.0 kDa polypeptide containing 227 amino acid residues.

Physical form

Lyophilized from 0.25x PBS + 1mM DTT.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sequence

MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF

Colourwhite
ManufacturerSIGMA-ALDRICH
Impurities﹤0.1 EU/µg endotoxin, tested
Formlyophilized
Assay≥98% (SDS-PAGE), ≥98% (HPLC)

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