IFN-gamma human,;, Animal-component free, recombinant, expressed in E. coli, >=98% (SDS-PAGE), >=98%

IFN-gamma human,;, Animal-component free, recombinant, expressed in E. coli, >=98% (SDS-PAGE), >=98%

Stock Code: 3593029
Manufacturer Part No: SRP3058-100UG
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Application

IFN-? (interferon ?)-human has been used for the generation of inflammatory M1 cells from human monocytic cell line, THP1.

Biochem/physiol Actions

IFN-? (interferon ?) signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. It can suppress the generation of anti-inflammatory cytokines and enhance the secretion of proinflammatory cytokines. Additionally, IFN-? stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK (natural killer) cells and neutrophils. It is responsible for tumor rejection and is capable of killing tumor cells by autophagy or apoptosis. IFN-? is also involved in Hepatitis E virus disease pathogenesis.

General description

IFN-? (interferon ?) is an acid-labile glycosylated interferon produced by CD4 and CD8 T lymphocytes as well as activated NK (natural killer) cells. IFN-? receptors are present in most immune cells, which respond to IFN-? signaling by increasing the surface expression of class I MHC (major histocompatibility complex) proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. The gene is mapped to human chromosome 12q15. Human IFN-? is species-specific and is biologically active only in human and primate cells. Recombinant human IFN-? is a 16.7kDa protein containing 143 amino acid residues.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.4.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sequence

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

Colourwhite to off-white
ManufacturerSIGMA-ALDRICH
Impurities﹤0.1 EU/µg endotoxin, tested
Formlyophilized
Assay≥98% (SDS-PAGE), ≥98% (HPLC)

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