Topo I (C651-765) (C terminal domain) human, recombinant, expressed in insect cells, >=80% (SDS-PAGE

Topo I (C651-765) (C terminal domain) human, recombinant, expressed in insect cells, >=80% (SDS-PAGE

Stock Code: 3592877
Manufacturer Part No: SRP2032-5UG
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Biochem/physiol Actions

Human DNA topoisomerase I catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, recombination, chromatin remodeling and other DNA or RNA templating activities. The C terminal domain of DNA topoisomerase I spanning from amino acids 713 to 765 is highly conserved and connected to the core domain by a poorly conserved linker domain (residues 636-713). An active site tyrosine has been characterized at position 723. Mutation of this tyrosine to phenylalanine at position 723 causes topo I to preferentially bind the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNA.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Colourclear colorless
ManufacturerSIGMA-ALDRICH
Formfrozen liquid
Assay≥80% (SDS-PAGE)

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