BirA, recombinant, expressed in E. coli, >=65% (SDS-PAGE)

BirA, recombinant, expressed in E. coli, >=65% (SDS-PAGE)

Stock Code: 3592786
Manufacturer Part No: SRP0417-100UG
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Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Biotin ligase BirA controls biotin synthesis in Escherichia coli. It sends biotin to metabolism. As a homodimer, it negatively controls the biotin synthetic operon. BirA also catalyzes post-translational biotinylation, by associating with biotin acceptor protein of acetyl-coenzyme A carboxylase. It can biotinylate human histones.

General description

Biotin ligase BirA (GenBank Accession No. AP012306 (3602298-3603260)) amino acids 2 – 321 (end) with His-FLAG-tag, MW = 37.1 kDa, expressed in an Escherichia coli cell expression system.

Physical form

Formulated in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% Glycerol.

ManufacturerSIGMA-ALDRICH
Storage Temp.−70°C
Formaqueous solution
Assay≥65% (SDS-PAGE)

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