T4 Beta-glucosyltransferase, recombinant, expressed in E. coli, >=83% (SDS-PAGE)

Stock Code: 3592785
Manufacturer Part No: SRP0416-100UG

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Descriptions

Application

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

Biochem/physiol Actions

T4-phage ?-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg²⁺, Mn²⁺ and Ca²⁺ activate this enzyme.

General description

T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.

Physical form

Formulated in 200 mM imidazole and 20% glycerol.